Tottenham have written to Professional Game Match Officials Limited (PGMOL) chief Howard Webb to raise their concerns over key refereeing decisions.
Sources have told BBC Sport the correspondence is primarily focused on what the club views as inconsistent decisions that have led to goals being allowed or disallowed as a result of contact inside the penalty area.
Spurs believe they have been on the wrong end of a number of these key incidents this season.
During Tottenham's 4-1 home loss to north London rivals Arsenal on 22 February, striker Randal Kolo Muani had a goal - which would have levelled the score at 2-2 - ruled out after the officials adjudged the France international had pushed opposing defender Gabriel Magalhaes in the back just moments before slotting beyond David Raya.